HCV usually suspected by the detection of HCV antibodies. Anti-HCV assays have several disadvantages as the lack of sensitivity of detection in the early window period after infection, the inability to distinguish between different stages of infection. Recombinant immunoblot assays, also have several disadvantages, such as being difficult to perform and having a high percentage of indeterminate results and a high cost.The HCV RNA assay is a reliable method but needs technical skill and may also result in false positivity because of contamination; it is time intensive and more expensive.